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KMID : 0895420040140030203
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Journal of Korean Society of Occupational and Enviromental Hygiene
2004 Volume.14 No. 3 p.203 ~ p.212
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Effects of Trichloroethlene on the Placental Function and Reporduction in Rat
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Kang Jeong-Hak
Lee Chae-Kwan
Moon Deog-Hwan
Son Byung-Chul
Kim Dae-Hwan
Lee Chang-Hee
Kim Hwi-Dong
Kim Jung-Won
Lee Chae-Un
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Abstract
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This study aimed at investigating the toxic effects of trichloroethylene (TCE) on the placental function and reproduction in rat. For these study placental prolactingrowth hormone (PRL-GH) family gene expression, placental trophoblast cell frequency and reproductive data were analyzed. Pregnancy of the Sprague-Dawley rats were checked by the presence of the copulatory plug or sperm in the vaginal smear and defined as the pregnant day (PD) 0. The pregnant rats were divided into the three groups. The control group was intraperitoneally injected with sesame oil. The remaining groups were injected with 100 or 500mg/kg B.W/day of TCE resuspended with sesame oil from PD 7-11 or 16-20. Rats were sacrificed at PD 11 and 20. mRNA levels of PRL-GH family and Pit-1a, b isotype genes were analyzed by Northern blot hybridization and Reverse transcription-polymerase chain reaction. Hormone concentration was analyzed by radioimmunoassay. Frequency of placental trophoblast cells were observed by histochemical study. Reproductive data such as placental and fetal weights pregnancy period, and litter size were surveyed at PD 20 and after birth. Statistical analysis was carried out by the SAS program (version 8.1). mRNA levels of PRL-GH family genes such as placental lactogens and placental prolactin like proteins were reduced by TCE administration. mRNA level of Pit-1a, b isotype genes that induce the expression of PRL-GH family genes were also reduced by TCE administration. Placental lactogen II concentration in the placenta, fetus and maternal blood were decreased by TCE administration. Exposure to a high dose of TCE reduced the frequency of the spongiotrophoblast cell in junction zone. Reproductive data such as placental and fetal weights, litter size were reduced, and pregnancy period was extended in the TCE exposed group than control. These data suggested that TCE disrupts the ordered functions of placenta and these effects lead to the reproductive disorder in rat.
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KEYWORD
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Trichloroethylene, Pit-1 gene, placenta, PRL-GH family gene, reproduction
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